Telomere Length Differences upon Keratinization and its Variations in Normal Human Epidermal Keratinocytes

Cultured Normal Human Epidermal Keratinocytes

H]glucosamine has been used to label metabolically keratinocyte cell-surface glycoconjugates. The major labelled bands identified on sodium dodecyl sulphate/polyacrylamide gels had apparent molecular weights of greater than 250000, and 150000-80000. Most of these components were trypsin-sensitive, indicating that the label was protein-bound. Some of the labelled components were shown to be prot...

Telomere Length Variations and Chromosome Fusions in Human Tumor Cells

PROCEEDINGS DEDICATION To the faculty and staff, who through generosity and passion invite undergraduates to participate in the knowledge-making enterprise, and to the students who so eagerly take up that challenge.

Isolation and in vitro cultivation of human skin Keratinocytes and preparation of epidermal sheet

Effect of hyaluronan tetrasaccharides on epidermal differentiation in normal human epidermal keratinocytes.

OBJECTIVE Hyaluronan (HA) plays a role in keratinocyte proliferation and differentiation. In addition, HA has been shown to have different biological activities depending on its molecular weight. It has been reported that HA-mediated CD44 activation regulates keratinocyte differentiation. Therefore, the aim of this study was to investigate the influence of HA tetrasaccharides (HA4) on the regul...

Sulfur-containing Proteins and Epidermal Keratinization

Sites of synthesis of sulfur-containing protein in keratinizing epithelium have been studied by radioautography after injection of sulfur-labeled cystine. Bern et al. (3) demonstrated concentration of silver grains immediately below the cornified layer, suggesting that much of sulfur is added at the final stages of keratinization as observed in hair. In contrast, B6langer (4) reported that prot...